Blot hybridisation analysis of genomic DNA.

نویسندگان

  • S Vandenplas
  • I Wiid
  • A Grobler-Rabie
  • K Brebner
  • M Ricketts
  • G Wållis
  • A Bester
  • C Boyd
  • C Måthew
چکیده

Restriction endonuclease analysis of specific gene sequences is proving to be a valuable technique for characterisation and diagnosis of inherited disorders. This paper describes detailed protocols for isolation, restriction, and blot hybridisation of genomic DNA. Problems and alternatives in the procedure are discussed and a troubleshooting guide has been provided to help rectify faults.

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عنوان ژورنال:
  • Journal of medical genetics

دوره 21 3  شماره 

صفحات  -

تاریخ انتشار 1984